Ritu Rathi

4 Questions 11 Answers 0 Followers

Questions related from Ritu Rathi

Can somebody help me with Methods/Assay to identify small molecules (present inside human system) binding with my protein of interest?

I am looking for methods that can help me in identifying natural small molecules binding with my protein of interest. Thanks in advance.

12 December 2017 9,080 2 View

Protein is not entering into gel (8%) from wells after DSS cross-linking?

Hi everyone, I am trying DSS crosslinking for my protein. My protein is expected to make trimer or hexamer with Ub. To prove this, I am crosslinking my protein with DSS. I tried crosslinking for...

09 September 2017 3,437 6 View

Can anyone please explain me reason for no change in UV or no peak during protein purification with AKTA prime plus (please check question detail) ?

I was working with GE superdex 200 column on AKTA prime plus system. I cleaned my column with NaOH, water and then I equibrilated it with buffer. During purification I saw no change in UV, it...

09 September 2017 7,807 6 View

Did anyone observe these type of sphere shaped crystals ( or may be spherulites). How can I improve or use them to get good shaped crystal ?

I'm trying to crystallize a protein and I have got sphere crystal almost in 10 well with different conditions (approx. 10-15 in each well). Could you please suggest me what are these and what to...

05 May 2017 4,700 3 View