Hello everyone
I am working in mango micropropagation through nucellar embryogenesis in Ratna (Mono-embryonic). Among 722 developed plants. I found one off type plant, the morphology of one leaf quite different (but other leaves are normal). During in vitro phase, yellow patches were observed on dorsal side surrounding the midrib, but no out growth was observed on ventral side. But after 2.5 months of hardening period, (total 4.5 month old plan), wheat like of outgrowth was observed ventral side (opposite) of yellow patches. Now gradually it’s turning to brown.
Can anyone suggest the exact cause of this symptom?
It looks like leaf curl, which is a fungal infection (Taphrina sp.)
Have you checked the ploidy level? In other dicot species haploids show this phenotype...
Hi, may I know how you establish aseptic culture for mango? Because I am working on mango also and all explants died due to browning after surface sterilization...
Dear Dr. Luis Carlos da Silva Ramos
Thanks for your opinion. I not yet checked its lopidy level.
Dear Eyu Chan Hong
Could you please tell me, what pathway you are using for mango micropropagation; i mean Somatic embryogenesis or axillary bud induction (using nodal explant)? Then i can help you.
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