What are the technical steps to calculate the dilution factor for my ELISA samples (proteins or Growth factors in plasma) so I am sure I don't get min or max values in the readout results?
The dilution factor for ELISA samples will depend on the type of sample being used. Generally, samples are diluted from 1:50 to 1:5,000. The exact dilution factor should be determined based on the specific sample type and desired outcome.
Serial dilutions are used in ELISA. Once you do it for your sample type a few times you can do a smaller range of dilutions, however it's good practice to run multiple dilutions every time you do your ELISA
There are no exact method for that, but you can calculate theoretically by anticipating the concentration of samples. Samples can pe any types. Better mention the sample type for better understanding.