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What are the technical steps to calculate the dilution factor for my ELISA samples (proteins or Growth factors in plasma) so I am sure I don't get min or max values in the readout results?
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Recently in one of our tests we identified a cellular protein expressed without any mRNA expression of the same protein intracellularly. What could be possible explanation? Kindly add possible...
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If you want to prove the activation of the intracellular Wnt/b-catenin pathway, would an ELISA testing for the levels of intracellular phospho-b-catenin (or a ration of phospo/total-b-catenin) be...
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If I would like to freeze a protein supernatant for later testing with ELISA. Is this possible? Would the de-freezing damage the proteins? What are the ideal conditions/protocol?
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If you test an expression of target gene per PCR and normalize it against two reference genes indepenantly. How do you report the results and conduct the statistical analysis for the expression (...
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Dear community, What would you recommend as suitable post-hoc paired test (corrected for multiple testing) to be used following the Friedman test? on SPSS?
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