6 Questions 4 Answers 0 Followers
Questions related from Karim Mohamed Fawzy El-Sayed
What are the technical steps to calculate the dilution factor for my ELISA samples (proteins or Growth factors in plasma) so I am sure I don't get min or max values in the readout results?
20 April 2023 660 3 View
Recently in one of our tests we identified a cellular protein expressed without any mRNA expression of the same protein intracellularly. What could be possible explanation? Kindly add possible...
11 April 2023 4,488 6 View
If you want to prove the activation of the intracellular Wnt/b-catenin pathway, would an ELISA testing for the levels of intracellular phospho-b-catenin (or a ration of phospo/total-b-catenin) be...
04 August 2021 9,607 3 View
If I would like to freeze a protein supernatant for later testing with ELISA. Is this possible? Would the de-freezing damage the proteins? What are the ideal conditions/protocol?
30 July 2021 8,558 9 View
If you test an expression of target gene per PCR and normalize it against two reference genes indepenantly. How do you report the results and conduct the statistical analysis for the expression (...
31 January 2019 3,040 1 View
Dear community, What would you recommend as suitable post-hoc paired test (corrected for multiple testing) to be used following the Friedman test? on SPSS?
01 January 1970 4,380 8 View
