Hello there Azlan.

In my opinion, I wouldn't consider those to be 24000 ppm solutions.

If I were you, I would homogenize the peel in the solvent prior to the MAE with a homogenizer: weighing the 1.2g to an Erlenmeyer, add 50mL of water, 30 s of homogenization and then submit it to the MW. Post-extraction filtration can be performed also, if required.

In some samples 30 s might not be enough so use n times to assure homogeneity.

Keep in mind to not use more than 60 s each time as the tip starts to heat and might hinder your results.

Best of luck,

Filipe

Hi Filipe

For this case, this peel is not fully dissolved into the solvent extraction (water). I''m also used homogenizer to separate between supernatant and sediment after extraction using MAE. Is not easy to get the real value of this concentration since the sample not fully dissolved

Can we assume this solution is (24mg/ml=24000ppm)? that why I'm not apply this solution extraction into rotary evaporator since this solution already concentrate. if still proceed with rotary evaporator, this sample can be too concentrate, thus how many dilution need to do for bioactive compounds analysis, dilution factor will be create error effect and this error will make our data analysis is not really precise.

thanks

Hello again Azlan.

You're probably using a centrifuge to separate your both "phases" of your solution and not a homogenizer.

A homogenizer would grind the bulk of your samples and allow the solvent to better extract your samples and should be done prior to submitting to MAE.

Best of luck,

Filipe