Does anyone know how long the catalyst-chromophore reagent 4-bromophenacyl bromide can be stored in an acetonitrile solution with 18-crown-6? Does the solution have to be used the same day, or within the week?
Thanks. I'll take that into account when I decide how much to make.
We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...
01 July 2024 1,983 0 View
We have been working with an inhibitor that clearly and consistently reduces an enzymes product by about 25-30%. However in trying to elucidate the mechanism using Michaelis-Menten and...
14 April 2024 4,921 5 View
I have been having troubles with column purification (for DNA) recently. I am assuming that the reason is the residual ethanol. I used to centrifuge at max speed for 2 minutes to remove residual...
30 November 2023 3,211 1 View
We have been studying the effects of some inhibitors on lysozyme activity. When we look at a graph of product (fluorescence) over time we see a clear inhibition of the enzyme activity that...
05 November 2023 9,834 1 View
I didn't deal with phage methods before. for that, I don't have the experience or the imagination so when I read "Isolate the top agar with the phage titre, then centrifuge" within procedures. I...
10 September 2022 7,777 5 View
Hi, I am interested in the polysaccharide composition of wheat and oat bran. I assume that cellulose, arabinoxylans, mixed-linkage glucans and possibly some starch will be present, because these...
06 July 2022 4,882 0 View
I have to replace it and I want to make sure I'm still using the same method
09 December 2021 8,152 3 View
Dear professionals, i run ANOVA test in SPSS and I found that three independent variables were not significant. I exclude them and re-run the test again ANOVA Test. the result are almost the...
25 October 2021 4,005 4 View
We have been using the Enz Check lysozyme assay and are assuming the cells are not intact but in pieces. However over time and especially with vortexing or pipetting to mix substrate, I would...
02 October 2021 3,491 1 View
Does anyone know of a reducing ends assay which can be used on pectins without degrading the pectin backbone? The DNS assay, which I have been using for pectinase assays, contains strong alkali.
07 June 2021 4,688 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View