Can we replaced PEG by any other fusible material when we preparing solid dispersion by melting-solvent method?

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Has anyone used the new E1 Clip tip digital pipettes?

We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...

01 July 2024 1,983 0 View

Why would Vmax and Km not change when inhibitor shows clear reduction in activity?

We have been working with an inhibitor that clearly and consistently reduces an enzymes product by about 25-30%. However in trying to elucidate the mechanism using Michaelis-Menten and...

14 April 2024 4,921 5 View

To remove residual ethanol for column purification, would using a desiccator work?

I have been having troubles with column purification (for DNA) recently. I am assuming that the reason is the residual ethanol. I used to centrifuge at max speed for 2 minutes to remove residual...

30 November 2023 3,211 1 View

Can you help with question about MIchaelis Menten graphs for Lysozyme activity?

We have been studying the effects of some inhibitors on lysozyme activity. When we look at a graph of product (fluorescence) over time we see a clear inhibition of the enzyme activity that...

05 November 2023 9,834 1 View

How to Isolate Top agar with already phage titre?

I didn't deal with phage methods before. for that, I don't have the experience or the imagination so when I read "Isolate the top agar with the phage titre, then centrifuge" within procedures. I...

10 September 2022 7,777 5 View

Is there published research about the polysaccharide composition of cereal brans?

Hi, I am interested in the polysaccharide composition of wheat and oat bran. I assume that cellulose, arabinoxylans, mixed-linkage glucans and possibly some starch will be present, because these...

06 July 2022 4,881 0 View

How i can interprete ANOVA info in SPSS?

Dear professionals, i run ANOVA test in SPSS and I found that three independent variables were not significant. I exclude them and re-run the test again ANOVA Test. the result are almost the...

25 October 2021 4,005 4 View

Are the Micrococcus lysodeikticus cells in the enz check lysozyme assay intact?

We have been using the Enz Check lysozyme assay and are assuming the cells are not intact but in pieces. However over time and especially with vortexing or pipetting to mix substrate, I would...

02 October 2021 3,491 1 View

Is there a reducing ends assay suitable for pectin?

Does anyone know of a reducing ends assay which can be used on pectins without degrading the pectin backbone? The DNS assay, which I have been using for pectinase assays, contains strong alkali.

07 June 2021 4,688 1 View

Where can I find Korean-English and Japanese-English Speakers to recruit for my research study?

I am doing a research study looking at Korean-English and Japanese-English bilingual individuals as my participants. I am trying to see if there are large communities of Korean or Japanese...

25 March 2021 2,990 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,936 1 View

How can I define dispersion for salt in CMG or Eclipse?

How can I define dispersion for salt in Eclipse in a low salinity water flooding code? how can I active interactions between tracer and polymer in Eclipse? Please let me know if you have any idea.

05 August 2024 1,218 1 View

London dispersion in CCl4 vs the dipole-dipoles force strength in CHCl3?

How do you tie-break and rank these two in their strength?

03 August 2024 4,471 1 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,225 1 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,160 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to conduct the NMR study of a nano-composite film?

Solid state NMR study

03 July 2024 421 1 View

the increase in ΔS0 from AC1 to AC2 (67 J/mol to 143 J/mol) ?

If we compare the values, the increase in ΔS0 from AC1 to AC2 (67 J/mol to 143 J/mol) indicates that the amount of adsorption is higher for AC2 compared to AC1. This increase in entropy means that...

10 June 2024 9,287 0 View

Which dispersion correction method should be used with MO6-L/6-311+G for the optimization of Ionic liquid?

since dispersion correction GD3BJ is incompatible with minnesota, which dispersion correction should be added in calculation.

05 June 2024 8,004 1 View

As an etch process engineer, which books would be helpful for work?

Hello, I am an engineer who deals with etching processes. I'm trying to study the etching process and would like to ask which book would be good to study. I graduated from the Electronics...

05 June 2024 4,977 2 View