Is there a reducing ends assay suitable for pectin?

08 June 2021 1 5K Report

Does anyone know of a reducing ends assay which can be used on pectins without degrading the pectin backbone? The DNS assay, which I have been using for pectinase assays, contains strong alkali.

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://academic.oup.com/jaoac/article/84/6/1851/5656868

https://www.semanticscholar.org/paper/Enzyme-assay-for-identification-of-pectin-and-based-Hansen-Truesen/aaf18b3d79dc12d4874d4e3d714cae0ae539443f

https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/268/630/pectinesterase.pdf

Thanks

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