We have been studying the effects of some inhibitors on lysozyme activity. When we look at a graph of product (fluorescence) over time we see a clear inhibition of the enzyme activity that indicates a non-competitive inhibitor. However when we assay a serial dilution of substrate concentration in respect to product we do not get a rectangular hyperbolic graph. Instead we get a line that does not level off? We have decreased the enzyme 10X and used the highest concentration of cells (substrate) that we can but still can see it level off? when we plot a LIneweaver Burk plot the line does not incept the y axis but instead goes through the 0,0 point on the graph? Any suggestions?