What would be the best survey design and stat. analysis for my research- impact of pandemic on pro-conservation and pro-environmental behaviours?

Ive started to design the survey with demographics, I have a list of pro-environmental and conservation behaviours which i plan to ask if they have changed since the pandemic. I think i'll use...

15 February 2021 4,204 3 View

What is the meaning of the broad oxidation peak and sharp reduction peak in CV?

Hi, I'm working on cyclic voltammetry (CV) using a chemical. The result was hard to explain as the CV showed a broad oxidation peak and a sharp reduction peak. Additionally, those peaks were...

07 December 2020 8,813 2 View

What company or university lab do you recommend to sequence specific bacterial species from a human microbiome sample?

We are looking for a lab to send human microbiome samples to that can quantify specific bacterial types from . We need a company you have found to be reliable, cost effective and reputable.

07 October 2019 198 4 View

SDS PAGE troubleshooting?

I have been having trouble with SDS PAGE--as you can see there are small bumps in 2nd and 7th (2nd and 8th as well) well. This is precasted gel 4-12%--80V for first 10 min and 120V for 80min. I...

09 September 2019 7,385 8 View

Neuroscience and Computer Science- Coding?

I'd love to hear your opinions/experiences!!! AT ALL LEVELS! What is the best computer program code (to further pursue) for neuroscience models? From chemical/cellular neuroscience to cog...

15 April 2019 3,172 3 View

Mixed Model ANOVA or Multiple Paired Sample T test?

I'm currently working on my research and this is the design: The aim of the research is to investigate the effectiveness of mindfulness on subjective wellbeing and perceived stress. So, 100...

27 July 2018 3,177 3 View

Extracting DNA, how to increase yield from FFPE fatty tissue?

I am extracting DNA and RNA from FFPE tissue, matched colorectal metastasis, primary tumour and matched normal tissue. (RNA is complete, with the DNA I am going on to do exome sequencing and...

02 July 2018 427 3 View

Are there any reagents that work well to directly conjugate fluorophores to antibodies of the IgM isotype?

We are developing a panel of antibodies for flow cytometry but several of the antibodies are of the IgM isotype. We are trying to find a kit or reagent to directly conjugate the antibodies to...

26 June 2018 465 2 View

How can I take images from Olympus fluorescence microscope after saving them on the computer of the microscope?

When I saved the images on the computer of the microscope, they appeared blue for DAPI stained nuclei, and green for FITC stained cellular membranes. When I took the images and opened them on my...

04 January 2018 1,299 3 View

Has anyone figured out a way to avoid condensation on 96 well plates in plate reader?

We are trying to assay bacteria growing at 37 C in plate reader over time and are having condensation problems. Any ideas?

25 April 2017 881 5 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View