Hello I am doing primary pulp cell culture and I get them from the mechanical method. better known as the overgrowth method. the problem has been two: adhesion and another is that even if there is adhesion in one well or another there is no proliferation of cells.
another issue is the enzymatic method that we do with collagenase type I and it goes straight to the T25 flask, but the cells are not adhering to the bottom of the bottle. Any suggestion?
What type of cells are you trying to culture..is it bacterial analysis?
I'm trying to culture dental pulp cell, it is primary culture, extracted directly from pulp tissue
You might require regenerative medium for pulp cells..try searching for tideglucibe in Google for dental pulp culture
But I don't wanna regenerate, I wanna proliferate these cells, make sense to you? thank you for taking your time for helping me
Mesenchymal stem cells (MSCs) are attractive tools for tissue repair because of their differentiation capability and abundance in tissues. Virtually every tissue has tissue specific stem cells. Although, MSCs were first isolated in bone marrow, a decline is observed in using bone marrow MSCs while interestingly, an increasing trend is evident regarding exploration of various postnatal tissues as a source of MSCs. Growing evidence suggests a remarkable regenerative potential of MSCs from dental derived tissue such as Dental pulp stem cells (DPSCs) from impacted third molar,5 stem cells from apical papilla (SCAP)6 and stem cells from human exfoliated deciduous teeth (SHED).
Protocol for the extirpation of dental pulp tissue, in-vitro explant culture and propagation of dental pulp MSCs from permanent and deciduous human teeth. MSCs are comprised of heterogeneous population thus it is important to characterize them for the presence of stem cell sub-populations. Therefore, we plan to characterize these cells using classical MSCs markers and their regeneration potential in-vitro. We believe, the major benefits from the protocol that it could provide a reliable source of stem cells to be used in regeneration of damaged or diseased tissue or organs, generation of patient specific stem cells and iPSCs banking.
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