Could B16F10 cells survive in a serum-free conditions?

13 March 2017 1 5K Report

Please, I need isolate exosomes derived from B16F10 cells. Does a medium without FBS

Hi Isadora,

Best is to use exosome depleted FBS (commercially available), other option is to deplete exosome of FBS by yourself. Since to get exosome you will need to incubate cells in that medium at least 1 day, so cells might be already sick in that condition and could affect your result.

Goodluck!

Tugsuu

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