Hello,

has anyone experience with density centrifugation with yeast cells, especially with the isolation of the yeast plasma membrane and cell wall compartments?

I read some protocolls (for example: Chang et al. DOI: 10.1007/978-1-59745-261-8_10 IN Methods in molecular microbiology) but most times there results a mixture of mitochondria and plasma membrane. Does anyone have experience or other protocols to separate these.

Do you also know if I change the physiological changes of the membrane?

Best regards

Marco