5 Questions 8 Answers 0 Followers
Questions related from Marco Eigenfeld
Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...
29 December 2020 4,548 11 View
Hello everybody, can someone give me a hint how to unmix fluorescence data in GFP and autofluorescence from yeast cells in flow cytometry? Thanks a lot in advance
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Hello everyone, I try to purify a chitin binding domain in a native conformation in mono disperse suspension. After purification by use of a polyhistidine tag I dialyze the protein suspension in...
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Hi everyone, I want to have an advice for the analysis of flow cytometric analysis of cells by the use of fluorescence intensity and front scatter. I stained yeast cells and want to analyze the...
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Hello everybody, I want to analyze yeast cells in flow cytometry (excitation 488 nm) by use of a fluorescence indicator (GFP). I've got the problem, that autofluorescence of yeast cells is higher...
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