11 Questions 15 Answers 0 Followers
Questions related from Marco Eigenfeld
Hello fellow researchers, I am currently encountering significant challenges in a classical cloning procedure and am in need of your expertise. Here is a brief overview of my...
04 May 2024 7,697 5 View
Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...
29 December 2020 4,698 11 View
Hello everybody, can someone give me a hint how to unmix fluorescence data in GFP and autofluorescence from yeast cells in flow cytometry? Thanks a lot in advance
05 September 2019 6,072 2 View
Hello everyone, I try to purify a chitin binding domain in a native conformation in mono disperse suspension. After purification by use of a polyhistidine tag I dialyze the protein suspension in...
14 August 2019 2,979 6 View
Hi everyone, I want to have an advice for the analysis of flow cytometric analysis of cells by the use of fluorescence intensity and front scatter. I stained yeast cells and want to analyze the...
06 July 2019 9,115 3 View
Hello together, do you have some advice for a optimal GFP sequence for an expression in E. coli BL21 (DE3)? I want to create an Insert sequence including a SUMO protein in combination with a His...
14 March 2019 8,569 1 View
Hello, has anyone experience with density centrifugation with yeast cells, especially with the isolation of the yeast plasma membrane and cell wall compartments? I read some protocolls (for...
17 August 2018 6,830 0 View
Hello, can anyone help me in my problems? I want to measure the membrane fluidity and changes of that in yeast cells. So I started with the fluorescence dye DPH. But theoretically DPH has no...
02 August 2018 9,485 6 View
Hello everybody, I want to analyze yeast cells in flow cytometry (excitation 488 nm) by use of a fluorescence indicator (GFP). I've got the problem, that autofluorescence of yeast cells is higher...
01 January 1970 1,450 8 View
What experience have you made by measuring the yeast vitality? Common methods are the measurment of the intracellular pH with CFDA, BCECF-AM or the protein content by the use of PI. What...
01 January 1970 2,812 2 View
Dear members, I would like to ask for your opinions about the process of yeast cell aging. Which theory is in your opinion more trustful for explaining yeast cell string? Thanks for an...
01 January 1970 8,684 8 View
