Hi all
I am reading QIAGEN's article (https://www.qiagen.com/es/resources/faq?id=2d04d81f-ef1f-4d7e-aaa5-2f30767445b1&lang=en) about the delta-delta CT method of quantifying qPCR. It says that it would not require standard curves but then you have to perform a validation experiment to test the amplification efficiency but isnt that the same as a standard curve?