We want to investigate if it is possible to visualise IP3-activated compartiments and calcium signalling in neurons. Thank you for your answer. Best regards
I'm not an expert here, but I'm reasonably sure that using a straight GFP mouse probably wouldn't work because you would be expecting GFP to express in response to second-messenger activation. This part may be feasible, but you would also need the GFP expression to be somehow quenched fairly quickly once IP3 level dropped down. Basal IP3 activity in neurons may also give you a background signal that could confound your observations.
However, there is a FRET-based system that might do what you're looking for: http://www.ncbi.nlm.nih.gov/pubmed/16754959