I am asking for the ideal editing of sequences obtained using dye termination method. I have sequences for the internal transcribed spacer (ITS) of nuclear DNA (nrDNA). The sequences include bases labelled with N; from the trace file, I have tried to replace each N with the suitable base. Any further editing should be done with the sequences before doing the multiple sequences alignment particularly in regards to cutting the begin and the end of the sequence? And how it should be done? I am also would like to ask for online classifier for fungi based on its sequence. REGARDS

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