Dear all, anyone knows how to obtain pax7+/myoD- skeletal muscles progenitor cells (satellite cells) from mesenchymal stem cells?

Francesco Della Valle @Francesco-Della-Valle

26 February 2020 1 3K Report

Dear all, anyone knows how to obtain pax7+/myoD- skeletal muscles progenitor cells (satellite cells) from mesenchymal stem cells? i am looking for and induction protocol without using transcription factors over-expression.

thank you ;)

Ellen A G Chernoff

Have you tried Wnt3a treatment? it is critical in induction and maintenance of pax7, while inhibiting myoD, myogenin in segmental mesoderm.

https://reader.elsevier.com/reader/sd/pii/S0012160604000922?token=2D19C931B8C55298715389C6BAB46AA4BAB8AAF927E66953AD8496982EB3DE32D5F80AAAB4B779FDC350E3B2DC0449DF

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