Dear experts and colleagues,
I am facing a problem during making PASSIVE conjugation in Lateral Flow Assay(LFA).
The gold nanoparticle was bound to the wall of the tube too much and affect the result of the conjugation.
Do you have any experience in dealing with this problem?
1) Can I use the low protein binding tube instead of normal plastic tube?
2) Can I rinse the tube with DI water or something else? e.g. isopropyl alcohol. If yes, what is the concentration?
Thank you so much for your help!