Hello,
I have a question for designing the experiment, I am interested to design markers for insecticide resistance in an insect that its genome has not been sequenced. I am thinking to sequence the genome, do de novo assembly and then look for variants between tolerant and control insect. Is this sounds practical? How about going through transcriptomics instead?
Appreciate your comments, Mahnaz
According to my opinion, you should go for sequencing and trasnscriptome analysis only. Markers for insecticide resistance is not a practical idea. It can have several complexities as most of the insect has various group of genes conferring resistance. Insects have more number of generations per year, so mutation can occur earlier and other such complexities.
Mahnaz, I think Muhammad is correct, but what a whole genome sequence will potentially identify individual markers or markers in LD with these causal variants, which you could then investigate to see which genes these variants are associated with
Hi, Mahnaz. How is your work going? In my opinion, your could figure out some different expressed genes between resistance and sensitive insect after transcriptomics sequencing. In general, the ORF is incomplete after transcriptomics sequencing, so a whole genome sequence is a very powerful database for your to obtain the full length of specific gene and your next research.
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