I have a question about investigating insecticide ( Neonicoitnoids) resistance mechanism. We have populations of insects that are resistant and susceptible to insecticide, and we are interested to find out about the mechanism behind it. Any recommendation that how we can do it?
Looking forward to hearing your ideas.
Thanks
This will depend on your species and the funds that you have available.
If it is a widely studied species then the resistance conferring point mutations in the Nicotinic Acetylcholine receptor may be known and you can screen for these in your samples. You can then see if frequencies differ between colonies and perhaps field-collected samples of differing resistance. If the mutations are unknown in your species you may be able to sequence particular NAChr genes and locate potential target site mutations.
You can try synergist exposure prior to bioassays to indicate the potential involvement of particular detoxification families. If metabolic resistance is indicated you can then try RNASeq to identify upregulated genes. With that information you could knockdown the gene and quantify the effect on the phenotype.
Hello @ Mahnaz Kiani ,
i am also working on insecticides resistance mechanism in insects.
I agree with Craig S Wilding arguments...
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