I used Augustus to predict gene and I don't know how I should validate these predictions in galaxy?
Hi Mahnaz, could you give more details about your workflow? I am assuming you did full genome sequencing on species S; used Augusts to predict genes, and want to validate those genes using a Galaxy pipeline. Is this correct?
I entered the data to Arlequin and under Genetic structure, the AMOVA option is not active, is this related to input data? The other options are active and will run the analysis. I enclosed the file.
07 August 2019 1,701 4 View
Hello, I have ddRAD data for 8 populations (48 samples in total) and looking for resistance lined SNP in one of the population. I did run data on Stacks and got the final outputs which...
06 July 2019 7,544 2 View
I have a question about investigating insecticide ( Neonicoitnoids) resistance mechanism. We have populations of insects that are resistant and susceptible to insecticide, and we are interested...
07 August 2018 4,889 2 View
Hello, I have a question for designing the experiment, I am interested to design markers for insecticide resistance in an insect that its genome has not been sequenced. I am thinking to sequence...
08 September 2017 599 3 View
I have 3 sets of data for RNA-seq from 3 biological reps, and I am just wondering how I should compare reps together statistically to be sure that they are correlated. My preference is CLC. Thanks
11 December 2014 3,119 2 View
I'm kind of confused with data normalization in CLC, I Compared RNA-seq results after doing normalization in data or on an experiment using legacy version and also using original data and...
09 October 2014 702 6 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View