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In my ddPCR assay to check my specific edits the PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and Probe 2 (labelled with...
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I have designed a ddPCR assay to detect gene edits in my organism. The PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and...
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