In an in vitro xanthine oxidase inhibition assay where xanthine is the substrate, do I get a colored product?

16 March 2023 0 2K Report

The experiment uses spectrophotometer

Badges
Science topic

Similar topics
Microbiology
Assays

More Anmol Chettri's questions See All

Yeast carbon base (YCB) + agar + BSA?

I'm planning to perform BSA degradation using YCB and agar, but I need a protocol. Does anyone have one they'd be willing to share?

02 June 2024 6,885 8 View

DdPCR 3' non extendible probe query?

In my ddPCR assay to check my specific edits the PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and Probe 2 (labelled with...

14 March 2024 7,010 1 View

DdPCR Auto dg consumables?

Hello, Is there any alternative (cheaper) Bio-Rad AutoDG-compatible tips? The price for a pack of 20 boxes has become double in 2024. Thanks

12 February 2024 1,350 0 View

Impedance data interpretation?

I am currently researching microbial growth kinetics through impedance analysis. My experimental procedure involves measuring impedance every hour for a duration of 24 hours. I am seeking guidance...

28 January 2024 4,779 3 View

DdPCR Auto-DG query?

Hi, I am currently using Bio-Rad Automated Droplet generator. I am happy with the result. I get on average 19K to 21K droplets per reaction. However, I have also encountered 1 to 2 samples...

12 December 2023 9,091 3 View

APC for PUBMED indexing ? Ethical ?

Most Pubmed indexed journals are charging Article processing fees / editing fees of 250-1250 $ making it impossible for students and young physicians to publish reseach in these journals . Isnt...

10 December 2023 2,924 3 View

Detection of gene edits using ddPCR?

I have designed a ddPCR assay to detect gene edits in my organism. The PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and...

29 November 2023 1,506 10 View

Which Polarisation is better?

Polarisation in Piezoelectrics The more the better or the less the better?

12 November 2023 8,099 0 View

Best tutorial for Qupath?

Hi, I am getting started with confocal image analysis in Qupath. I need to know if there are any good tutorial or seminar available online for researchers like ne to get started with. I have been...

13 September 2023 6,240 0 View

How to resolve this error while running UMAT in ABAQUS ?

Abaqus JOB Job-1 Abaqus 6.14-1 Abaqus License Manager checked out the following licenses: Abaqus/Standard checked out 5 tokens from Flexnet server abqs.iitkgp.ac.in.

21 August 2023 8,564 0 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Why applying a saved template of a bar chart in a different/new one doesn't take effect in SPSS?

Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...

29 July 2024 1,981 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View