How to calculate the gas composition for a heat treatment where a very low oxygen partial pressure is needed?

I'm interested in a selective oxidation in an austenitic stainess steel so that Chromia rather then ferrous oxide is formed on top of my metal. I know I should have a value of pO2 around...

16 December 2020 8,541 7 View

Why do protein and mRNA levels not always correlate with each other?

Hello, I have performed WB analysis and qPCR analysis to analyze the protein level and the rispective gene relative expression of mTOR, GBA1, LC3 genes in brain of mutant mice. I observed a...

01 December 2020 4,486 2 View

Factor analysis with idependent qualitative variables and dependent quantitative variables ?

Hello, I have a set of independent qualitative variables related to households (type of house, type of windows, presence of pets, ...), and dependent quantitative variables (number of arthropods...

19 April 2020 1,788 11 View

Why does LC3-II not increase after CQ treatment in vivo?

I have administered CQ treatment (50 mg/kg) in twelve-months-old C57BL/6J mice to assess autophagic flux. I validated this protocol on 3-months-old mice first, obtaining an accumulation of LC3II...

05 April 2020 5,018 3 View

What could be the advantages of annealing BaTiO3 in vacuum?

I am evaluating if annealing barium titanate in vacuum could have any benefits.

27 January 2020 4,329 1 View

Cas9 containing plasmid toxic for bacteria?

Hello everyone, I am struggling to obtain an overnight/culture for E.coli Stbl3 bacteria transformed with a plasmid containing the Cas9 enzyme and a GFP donor. Although the bacteria grow well in...

19 January 2020 2,245 3 View

Why lentiviral transduction doesn't work?

Hi everybody. I have a problem trying to overexpress by plasmid the gene of my interest (ffar4 for GPR120). I'm able to infect T293 (they appear totally green), then i filtered and centrifuged the...

28 July 2019 5,831 3 View

Experience with service for gene expression with microarray ?

I am looking for a company which can provide a service for several microarrays for gene expression and subsequent results analyses. Does anyone have any experience to share? E.g. Recommending or...

25 March 2019 1,015 3 View

Heat killing of Bordetella species?

13 March 2018 6,065 2 View

How simulate the behavior of a reinforced concrete beam with a B21 element type?

Hi everyone, I am modelling a reinforced beam under impact load. I chose to model it with element type B21. Any suggestion about how to define material's property in order to simulate...

31 January 2018 7,908 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View