17 October 2022 1 518 Report

Can anyone tell me what is the luciferin mostly used for in vivo imaging: D-Luciferin potassium salt? or D-Luciferin sodium salt?, who do you buy from?

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How to count metastatic nodule in slide?

how to count metastatic nodule in slides stained with H&E. different sections have different number of nodules. Should I add all or should I report the section that showed the highest number?

05 March 2024 4,640 2 View

I am trying to develop doxorubicin resistant cells. How to store and for how long the doxorubicin solution?

trying to develop doxorubicin resistant cells. How to store and for how long the doxorubicin solution?

16 January 2024 7,273 0 View

Could anybody please share doxorubicin resistant breast cancer cells with me?

I need doxorubicin resistant breast cancer cells (MDA-MB-231, 4T1 preference). It will take several months to develop the resistant strains. I will be grateful for ever if any of you is willing...

26 June 2023 6,026 1 View

Why my 4T1 breast cancer cells are not growing?

I have been trying to proliferate cells for almost 4 weeks culturing 4T1 cells in RPMI 1640 medium supplemented as recommended by the ATCC (10% FBS, 5% antibiotic). Started with a frozen vial...

30 May 2022 7,133 3 View

What concentrations of blasticidin-s have people used when selecting MKN-45 cells?

I'm trying to generate a kill curve in MKN-45 cells. I'm going to generate a stable KO in this line. I've found the cells detach at low concentrations of blasticidin-s. 2 ug/mL. I know that MKN-45...

27 September 2021 6,020 0 View

How to improve a Daphnia culture that is dying (without a clear reason?

Greetings, I am cultivating Daphnia magna for a while now and after a sudden change in the temperature (to 17 degrees during a weekend) they have not been able to recover. They are still able to...

01 May 2019 8,741 9 View

What is the role of IL-4 in generation of BM-DCs?

I see protocols for generation of mouse bone marrow derived macrophages generated with just GM-CSF or GM-CSF plus IL-4. Can someone tell me what role the added IL-4 plays in generation of these cells?

13 April 2019 3,424 2 View

Should I look at BCL-2 in whole cell or subcellular fraction?

I'm looking at BCL-2 in cultured cells. I'm comparing treatments that appear to inhibit apoptosis. I'd like to look at BCL-2 levels in whole cell lysates, but I'm wondering if I should be looking...

06 November 2017 5,562 3 View

Anyone knows whether is possible to perform an LST on frozen splenocytes?

I would like to check for T cell proliferation upon specific stimuli in mouse splenocytes. I know that it is possible to FACS on frozen splenocytes and look at T cell activation therefore I was...

24 February 2016 7,395 0 View

What kind of interventions on empathy or compassion are effective for health students?

Dear All: Thank you in advance for your help. I am doing my report (systematic review) on this topic, but I would like to centre it in medical and dental postgraduate students. Most of the studies...

30 June 2015 3,030 8 View

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Is it possible for the soil pH to be around 8, but with negligible amounts of carbonates, bicarbonates and exchangeable sodium?

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