I'm trying to generate a kill curve in MKN-45 cells. I'm going to generate a stable KO in this line. I've found the cells detach at low concentrations of blasticidin-s. 2 ug/mL. I know that MKN-45 cells can grow in suspension too. I counted the cells using a ViCell and got good viability for all my samples (2-10 ug/mL), but when I counted them on a Nexcelon instrument using AO/PI I found the cells were mostly dead (20% viability acros the board). Naturally I'm going to titrate lower with my blasticidin concentrations, but was wondering if anybody observed something similar before.