Can I perform simultaneous pectinase, cutinase and cellulase assay using fruit peels??
I want to increase the number of conformations which is by default 10. I want to get 100 conformations of a single ligand. Is it possible using autodock vina or any other software?
11 April 2024 8,736 2 View
Hello there! I am trying to dock ZIF-8 (MOF) with the drug doxorubicin using PyRx and discovery studio for visualization of results. My queries are: 1. When I convert .cif file to .pdb using...
27 March 2024 2,007 4 View
Protocol for Screening Low-Density Polyethylene (LDPE)–Degrading Soil Fungi Isolated from Urban Waste Dumping Sites
08 October 2023 6,968 1 View
I have data in the form of graphs and peaks and I want to find Tm and Tg from DSC/TGA graphs I have. Similarly, I have to perform ANOVA and FTIR analysis. Can OriginPro (trial version) perform...
10 July 2023 2,138 0 View
I have the graphs showing DSC-TGA simultaneously but I want to find the glass transition temperature and degree of crystallinity too. Is there some way to calculate or find them from the graphs or...
04 July 2023 3,222 5 View
I want to know whether HDPE is used as labels on plastic bottles of beverages like coke, Pepsi, 7UP, etc.
16 June 2023 1,525 1 View
I was using para nitrophenol acetate for an esterase test in a microtiter plate. The reaction contained just crude enzyme, p-NPA, and also Tris-HCl buffer (pH8). The final product was p-NP(para...
08 June 2023 7,189 0 View
I am performing an esterase assay with cell-free supernatant, and I want to reuse these plates after washing them with distilled water and then, bleach solution. Any suggestion? Can I perform this...
04 June 2023 1,523 0 View
Our Lab's FTIR knob broke. Can it give right results without the knob touching the polymer just by the diamond sensor in FTIR?
20 May 2023 7,520 2 View
I have made a standard curve of p-Nitrophenol concentrations. Now I want to apply the trendline equation on a huge dataset simultaneously to find the unknown concentrations from ODs. Which is the...
15 May 2023 879 0 View
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I would like to know the minimum amount of Cellulase enzyme that could be used for silage making.
03 August 2024 3,170 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?
27 July 2024 9,147 4 View