Sample size calculation?

07 September 2020 1,318 4 View

When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

13 October 2019 5,915 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

27 November 2018 9,085 3 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

11 February 2018 9,627 3 View

How do you calculate the total phenolic contents in a plant extract sample?

I have to find out the total phenolic contents in five different plant extracts. I have read many papers but am unable to understand the calculations how to express total phenolic contents as mg...

26 November 2014 5,255 56 View

Can anybody suggest a suitable region to design porcine specific qPCR assay, apart from mitochondrial genome?

I want to design species specific primers and probes for porcine, bovine, ovine, chicken etc, as mitochondrial genome is really conserved it's really difficult to design assays from MT genomes....

05 August 2014 8,873 3 View

What is the exact mechanism of selection of an X-chromosome for inactivation during female embryonic development?

It is said that a default state of the X-chromosome in female mammals is inactive and one of them is activated, as in case of females with three or four X-chromosomes, i.e. all except one remain...

26 September 2013 7,614 2 View

Can Anyone tell me how can we plot data for TGA curve through the data? i want to plot mass % v/s Temp but i dont have any data for mass %?

01 January 1970 6,946 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Looking for help in writing my research proposal ?

Looking for help in writing my research proposal in the following areas : HRIS / HR / Change management / Remote work Please get in touch with me if that sounds like your expertise. Thank you very...

08 February 2021 5,434 4 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View