Sample size calculation?

07 September 2020 1,318 4 View

When can E.coli secrete most of the extracelluar protein?

Hello, I'm doing optimization of an extracellular protein cloned in the pET26b vector along with PelB leader sequence. I want to know the best time at which cells can secrete most of the protein...

13 October 2019 5,915 6 View

How to confidently predict the 3D structure of a protein?

I have tried to predicted the 3D structure by using multiple online softwares like I-TASSAR, MUSTER, Swiss-Model and (PS)2 but predicted 3D structure from each software has bad quality and can not...

27 November 2018 9,085 3 View

How to do molecular dynamics simulations for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio??

Hello, I want to do simulations analysis for investigating the effect of non-synonymous mutations on protein structure through Discovery Studio. Kindly suggest me a protocol. Thanks

11 February 2018 9,627 3 View

How do you calculate the total phenolic contents in a plant extract sample?

I have to find out the total phenolic contents in five different plant extracts. I have read many papers but am unable to understand the calculations how to express total phenolic contents as mg...

26 November 2014 5,255 56 View

Can anybody suggest a suitable region to design porcine specific qPCR assay, apart from mitochondrial genome?

I want to design species specific primers and probes for porcine, bovine, ovine, chicken etc, as mitochondrial genome is really conserved it's really difficult to design assays from MT genomes....

05 August 2014 8,873 3 View

What is the exact mechanism of selection of an X-chromosome for inactivation during female embryonic development?

It is said that a default state of the X-chromosome in female mammals is inactive and one of them is activated, as in case of females with three or four X-chromosomes, i.e. all except one remain...

26 September 2013 7,614 2 View

Can Anyone tell me how can we plot data for TGA curve through the data? i want to plot mass % v/s Temp but i dont have any data for mass %?

01 January 1970 6,946 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View