I am performing an esterase assay with cell-free supernatant, and I want to reuse these plates after washing them with distilled water and then, bleach solution. Any suggestion? Can I perform this without sabotaging my results' credibility?
I want to increase the number of conformations which is by default 10. I want to get 100 conformations of a single ligand. Is it possible using autodock vina or any other software?
11 April 2024 8,736 2 View
Hello there! I am trying to dock ZIF-8 (MOF) with the drug doxorubicin using PyRx and discovery studio for visualization of results. My queries are: 1. When I convert .cif file to .pdb using...
27 March 2024 2,007 4 View
Protocol for Screening Low-Density Polyethylene (LDPE)–Degrading Soil Fungi Isolated from Urban Waste Dumping Sites
08 October 2023 6,968 1 View
I have data in the form of graphs and peaks and I want to find Tm and Tg from DSC/TGA graphs I have. Similarly, I have to perform ANOVA and FTIR analysis. Can OriginPro (trial version) perform...
10 July 2023 2,138 0 View
I have the graphs showing DSC-TGA simultaneously but I want to find the glass transition temperature and degree of crystallinity too. Is there some way to calculate or find them from the graphs or...
04 July 2023 3,222 5 View
I want to know whether HDPE is used as labels on plastic bottles of beverages like coke, Pepsi, 7UP, etc.
16 June 2023 1,525 1 View
I was using para nitrophenol acetate for an esterase test in a microtiter plate. The reaction contained just crude enzyme, p-NPA, and also Tris-HCl buffer (pH8). The final product was p-NP(para...
08 June 2023 7,189 0 View
Our Lab's FTIR knob broke. Can it give right results without the knob touching the polymer just by the diamond sensor in FTIR?
20 May 2023 7,520 2 View
I have made a standard curve of p-Nitrophenol concentrations. Now I want to apply the trendline equation on a huge dataset simultaneously to find the unknown concentrations from ODs. Which is the...
15 May 2023 879 0 View
I want to check if the PET, PVC, and pe have degraded in my liquid samples by using HPLC.
05 April 2023 4,979 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View