Can we reuse a microtiter plate previously used with supernatant only and not cells, again for supernatant only and for same test?

05 June 2023 0 2K Report

I am performing an esterase assay with cell-free supernatant, and I want to reuse these plates after washing them with distilled water and then, bleach solution. Any suggestion? Can I perform this without sabotaging my results' credibility?

Badges
Science topic

Similar topics
Microbiology
Assays

More Tehreem Ishtiaq's questions See All

Can autodock vina give more than 10 conformations with minimum binding energies?

I want to increase the number of conformations which is by default 10. I want to get 100 conformations of a single ligand. Is it possible using autodock vina or any other software?

11 April 2024 8,736 2 View

How to dock ZIF-8 (MOF) with drug doxorubicin?

Hello there! I am trying to dock ZIF-8 (MOF) with the drug doxorubicin using PyRx and discovery studio for visualization of results. My queries are: 1. When I convert .cif file to .pdb using...

27 March 2024 2,007 4 View

I need to access this protocol. Can someone please share?

Protocol for Screening Low-Density Polyethylene (LDPE)–Degrading Soil Fungi Isolated from Urban Waste Dumping Sites

08 October 2023 6,968 1 View

Can I use originPro for DSC and FTIR analyses?

I have data in the form of graphs and peaks and I want to find Tm and Tg from DSC/TGA graphs I have. Similarly, I have to perform ANOVA and FTIR analysis. Can OriginPro (trial version) perform...

10 July 2023 2,138 0 View

Can anyone tell me a software to analyze dsc-tga graphs?

I have the graphs showing DSC-TGA simultaneously but I want to find the glass transition temperature and degree of crystallinity too. Is there some way to calculate or find them from the graphs or...

04 July 2023 3,222 5 View

Can anyone please tell which type of plastic is used in preparation of labels used on plastic bottles of pespsi and 7UP etc.?

I want to know whether HDPE is used as labels on plastic bottles of beverages like coke, Pepsi, 7UP, etc.

16 June 2023 1,525 1 View

Does p-NitroPhenol acetate decomposes itself in Tris-HCl buffer without enzyme?

I was using para nitrophenol acetate for an esterase test in a microtiter plate. The reaction contained just crude enzyme, p-NPA, and also Tris-HCl buffer (pH8). The final product was p-NP(para...

08 June 2023 7,189 0 View

Is it necessary for the Bruker FTIR device knob to touch the polymer sample to give right results?

Our Lab's FTIR knob broke. Can it give right results without the knob touching the polymer just by the diamond sensor in FTIR?

20 May 2023 7,520 2 View

How the trendline of a standard graph is used to find the unknown concentration?

I have made a standard curve of p-Nitrophenol concentrations. Now I want to apply the trendline equation on a huge dataset simultaneously to find the unknown concentrations from ODs. Which is the...

15 May 2023 879 0 View

What would be the standards for HPLC analysis of polymers like PET, PVC and PE?

I want to check if the PET, PVC, and pe have degraded in my liquid samples by using HPLC.

05 April 2023 4,979 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View