Culture of fibroblst cells

More Hala O. Eltwisy's questions See All

What is the mechanism of atmospheric pressure plasma Jet (APPJ)?

In general, the characteristic of electric current as a function of voltage in argon plasma jet using dielectric barrier discharge in atmospheric pressure, jet length will increase by the increase...

16 July 2024 9,316 1 View

Please let me know where I can study the scaling effects of experimental studies on soil slopes in geotechnical engineering?

I am looking to simulate real-world landslides in a laboratory setting and measure parameters such as volumetric water content, pore water pressure development, seepage patterns, and ground...

23 June 2024 8,340 2 View

Peer support in adolescents with type 1 diabetes?

Type 1 diabetes, the effect of peer support in self care efficacy, emotional well being

27 May 2024 4,325 0 View

Dear all! Do you know simle protocol of conversion ssDNA to dsDNA (applied to ancient DNA)?

Without using commercial kits.

15 May 2024 844 0 View

Inquiry Regarding Supervision for Master's Research?

Hello everyone, I am currently pursuing my master's degree in Vaccine Production and Quality Control and I am actively seeking an internship opportunity to complement my studies. Could you please...

28 March 2024 6,483 2 View

In material studios which is better: focus most of my PC's 20 cores on one calculation at a time, or spread them among several calculations?

I am currently trying to run some calculations on material studios, and I noticed that allocating fewer cores makes the calculations take longer than when more cores are allocated. I have a total...

16 March 2024 9,265 1 View

Improved housing for seniors from the LGBQT+ community & the Roma and Traveller Communities?

Hi there, I have already put a question for a policy response. My team and I have been tasked with producing a Rapid Evidence Assessment (REA) on how housing can be improved for our senior...

15 March 2024 7,984 0 View

Can anyone give me examples of policies that have provided good housing options to senior citizens?

Hi there, I am studying for a part-time master's degree in public policy at DCU, Ireland. My group has been asked to prepare A rapid policy assesment on housing options for senior citizens. I...

01 March 2024 948 1 View

How do I claim authorship of an article that is wrongly ascribed to someone else?

Someone on researchgate is claiming two of my publications with the names edited to reflect and serve as his own. How do I claim the rightful authorship of the papers?

08 February 2024 4,787 2 View

How to induce inflammation in colon cancer cell lines?

I am trying to induce inflammation in colon cancer cell lines CACO2 and HT29 to study IBD. I have tried different concentrations of LPS for different timepoints and it is not working. does anyone...

23 January 2024 3,248 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Hiba Riyadh Al-abodi

Article Skin Punch Biopsy Explant Culture for Derivation of Primary ...

Khansaa Azeez Obayes Al-Husseini

The establishment of skin fibroblast strains provides a vehicle by which biochemical and genetic studies may be applied. In addition to providing genetic material to study the basis of disease, fibroblast cell lines established from skin biopsies may provide the investigator with a model in which to study specific disease states or normal physiology. This unit provides methods for the biopsy, establishment, and culture maintenance of skin fibroblasts to be used in further scientific study.