Hi,
How long do you usually rest cryopreserved dendritic cells after thawing? And what cell culture media does it need for resting? Thanks!
Hi, I'm analyzing some bulk RNAseq data from T cells under different conditions, and based on the RNAseq data, condition A shows higher expression of genes associated with effector functions...
01 July 2024 741 2 View
I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...
12 June 2024 1,714 4 View
I want to construct a nomogram for predicting the risk of fistula after esophageal surgery through comprehensive analysis of the currently published literature, and then verify its efficacy using...
25 May 2024 669 0 View
Does the catalytic activity of a lyase, such as a protease, change upon the formation of a μ-gelzyme?
21 May 2024 9,403 0 View
What is the principle of surface acrylation of enzyme protein ?
20 May 2024 9,428 0 View
Many literatures have followed Antras(2012)'Upsrteamness index of industry and combined trade data of firms to measure firms' GVC position. So, is there any better ideas or...
28 April 2024 2,607 4 View
Why papain is easily cross-linked by glutaraldehyde, while bovine serum albumin, keratinase is difficult to be cross-linked
17 April 2024 7,782 0 View
Does anyone know how to solve the magnetic structure by simulated annealing what are the specific steps, the official tutorial is not detailed, I do not know how to operate
26 March 2024 6,549 0 View
fullprof
26 March 2024 6,499 0 View
Dose and time of upregulation of CD86 and CD80 in RAW264.7 cells using LPS/IFNgamma?
24 March 2024 5,575 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,891 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,833 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,892 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View