Does anyone know how to solve the magnetic structure by simulated annealing what are the specific steps, the official tutorial is not detailed, I do not know how to operate
Hi, I'm analyzing some bulk RNAseq data from T cells under different conditions, and based on the RNAseq data, condition A shows higher expression of genes associated with effector functions...
01 July 2024 740 2 View
I want to construct a nomogram for predicting the risk of fistula after esophageal surgery through comprehensive analysis of the currently published literature, and then verify its efficacy using...
25 May 2024 669 0 View
fullprof
26 March 2024 6,499 0 View
Hi. I'm a new researcher trying to get ready to publish my first paper in the SCI Journal. Since my research is a combination of using psychological methods to screen obese and normal people...
18 March 2024 3,433 2 View
I'm running a VISSIM Com script using Python. My VISSIM version is 2021, and I'm using Python 3.7.4. When I execute the code in Visual Studio Code, the VISSIM window appears, and the simulation...
18 March 2024 5,400 0 View
After entering the space group, atomic coordinates, and propagation vector in SARah, I clicked on Calculate and encountered an unrecognized Jones symbol error pop-up. What made me anxious was that...
08 March 2024 9,566 1 View
I'm doing RNA dot blot with NC membrane. But its seems like something wrong with crosslinking. I'm wondering 1. If I need to use SSC buffer to treat with membrane before dotting? and what X of...
28 February 2024 4,704 1 View
Using TOF method of neutron diffraction data to process crystal phase, I have converted gsas prm file into irf, and added manual background, the data is doped, why is the Prague Summit missing...
26 February 2024 8,413 1 View
I am a beginner in FullPro, and now facing the problem of multi atom doping, how can I determine the doping position? Our current sample is doped with 3 atoms, and the doped element has 4 lattice...
19 February 2024 5,507 3 View
I have just started using Gsas software and would like to use it for magnetic refinement of samples. However, I am not sure how to use Gsas for the specific steps of magnetic refinement. Can...
19 February 2024 2,341 0 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,759 5 View
I have prepared Copper thin films by magnetron sputtering and the samples have been annealed at 100, 200 and 300 degree celsius. The variation of the rms roughness and all other AFM height related...
11 June 2024 9,772 1 View
I am doing my undergrad thesis that requires me to do PCR-RFLP analysis on DNA that I extracted from some blood samples. Initially, I was using the Qiagen mastermix for the optimization of my...
14 May 2024 4,369 4 View
Hi, I'm currently trying to generate a one-vector plasmid system for a knockout using CRISPR/Cas9. I'm using lentiCRISPRv2 (ampicillin-resistant) and following the GeCKO Target Guide Sequence...
29 April 2024 3,660 0 View
I recently designed the qrtpcr primers from the 3'utr of the plant's genomic DNA keeping in mind that the annealing temperature should be 60 degree. Once I received the primers first to optimise...
25 April 2024 5,934 3 View
Ligation Independent Cloning literally says no need Ligation, but it still needs ligase to anneal the fragment to vector. And the Protocol used T4 DNA Ligase for anneal process. What does this...
18 April 2024 1,493 3 View
Hi, I have been trying to decide on the best annealing temperature for the F/R primers using a sample. From left is the ladder, 61c, 63c, 65c, no template and the ladder. The expected product size...
11 April 2024 7,591 9 View
I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...
09 April 2024 4,485 5 View
When I simulate the NMOS model in ATHENA, when I want to anneal in nitrogen atmosphere after n+ ion implantation, the annealing operation is not possible when the annealing temperature is lower...
08 April 2024 2,798 0 View
Hi All, I am trying to amplify mitochondrial 16S gene for marine snails (Calliostomatidae) and other vetigastropods, but I only get primer dimers or nothing on the gel. These primers have worked...
03 April 2024 8,735 4 View