20 Questions 10 Answers 0 Followers
Questions related from Kun Yun
Hi, I'm analyzing some bulk RNAseq data from T cells under different conditions, and based on the RNAseq data, condition A shows higher expression of genes associated with effector functions...
02 July 2024 608 2 View
Hi, I have a general question about pathway analysis in scRNAseq. I'm under the impression that scientists pick pathways relevant to their study from the list of GO terms and present those in a...
14 February 2024 8,512 3 View
Hi, I'm planning to stimulate antigen specific T cells with CD3/CD28 microbeads for lentiviral transduction, and I'm also planning to stain TCRs after stimulation prior to TCR functional assays,...
08 February 2024 1,581 4 View
Hi, I did some scRNAseq as well as protein expression analysis on the same sample, however, the RNA level is not consistent with the protein level. How do people usually explain this piece of data...
02 November 2023 4,400 3 View
Hi, I'm using a very hydrophobic peptide for tetramer staining. The peptide can only be dissolved in DMSO, and then further diluted by PBS or water. I have tried 10, 5, 3 and 2 mg/mL as the stock...
08 August 2023 4,611 0 View
Hi, I have been trying to dissolve a very hydrophobic peptide (containing cysteine) with DMF, however, the solution looks very cloudy when I add DMF, and further dilutions with water doesn't help...
25 July 2023 6,187 2 View
Hi, I'm trying to block FcR on human macrophages in some in vitro cocultures with human T cells. Is there any FcR blocking reagents used in vitro culture? Thanks, Kun
10 July 2023 265 2 View
Hi, I have a basic question about cell engineering. Is it possible to load or engineer mammalian cells to produce certain chemicals?
26 June 2023 3,409 1 View
Hi, How long do you usually rest cryopreserved dendritic cells after thawing? And what cell culture media does it need for resting? Thanks!
19 June 2023 1,592 0 View
Hi, Has anybody ever seen stained cell population moves downwards compared to FMO?
14 June 2023 2,396 0 View
Hi, I'm trying to stimulate human T cells with magnetic beads coated with antibodies. I have streptavidin-coated beads as well as biotinylated antibodies. According to the vendor, they usually...
09 June 2023 6,913 4 View
Hi, I have been using HighScribe T7 polymerase kit for in vitro transcription from NEB, but the protocol gives a 20uL reaction with 1ug DNA template, which is not enough for my downstream...
31 May 2023 1,166 3 View
Hi, does anyone know how to find the DNA sequence encoding the scFv region of a specific antibody? Is there a library for these? Thanks!
05 April 2023 3,395 3 View
Hi, I'm using neutralizing monoclonal antibody to neutralize cytokine in cell culture. I added neutralizing antibody on Day 0, and measured the cytokine level 3 days later, but it seems that the...
14 March 2023 5,764 0 View
I am trying to expand CTLs in vitro and have noticed that many studies usually use irradiated PBMCs as feeder cells besides supplement with cytokines. What ratio of CTL to PBMCs does it usually...
19 December 2022 2,473 0 View
I read papers about in vitro transcribed RNA. What is the difference between in vitro transcribed RNA and commercially purchased RNA? Thanks
19 December 2022 2,375 2 View
Hi, I'm activating CD8 T cells by adding MHC-I restricted epitopes to whole PBMCs in vitro according to previous published studies. I noticed that cell feeding occurs 24 hrs after adding peptides...
19 October 2022 3,412 0 View
Hi, I'm trying to stain tumor specific TCR on T cells with either tetramer or dextramer. From what I know about these two techniques, dextramer helps to detect more tumor specific T cells due to...
12 October 2022 6,716 0 View
I'm trying to stimulate human PBMCs with peptides and hope to generate antigen specific CD8 T cells in vitro. However, some literatures used 1uM and some used 10 uM, so I'm wondering if there's...
23 September 2022 145 1 View
I ordered a bottle of D-biotin from ThermoFisher but can't find its expiration date anywhere on their website. Does anyone work with D-biotin (pH=10) and know how long its shelf life is? Thank you!
09 July 2022 6,528 0 View
