Self-docking and Cross-docking in terms of rigid vs flexible docking results tells that conformations are independent for every ligand and protein in the complex.
So is docking a right way of justifying or correlating with experimental results?
Rigid cross-docking gives high RMSDs in many cases. Then how can we rely on results generated for unknown compounds.
Does low RMSD of rigid self-docking validates the docking process for that protein? What are the possible errors for high RMSD in rigid self-docking and How to correct them?
Check this out:
https://www.researchgate.net/post/What_is_the_importance_rmsd_ub_and_rmsd_lb_in_docking_results
Article Is It Reliable to Take the Molecular Docking Top Scoring Pos...
It is also depends on which tools use and check minimum error by standard