How to incorporate a variable in damage model?

15 December 2020 7,284 2 View

Mathematical or computational modelling of protein oxidation?

I am looking for a research paper about the mathematical or computational modelling of protein oxidation (caused by reactive oxygen species).. I would really appreciate that if someone helps me...

17 November 2020 2,340 3 View

Can FEM model learn from experimental results?

I am looking for a research in which the finite element model was trained based on experimental results.

02 October 2020 9,277 19 View

Master thesis about the field of the luxury watch market?

18 September 2020 5,802 5 View

For connection between the bolt to the steel element, which kind of interaction should be considered on ABAQUS?

This design is to check for rupture in the steel element and the behavior of the bolt does not matter.

14 August 2020 6,580 2 View

Is it possible for anybody to help me for fix bridge modeling in OPENSEES?

I modeled a two-span concrete box girder bridge base on FHWA design specification, and now I recognize a problem in modeling because my first period is higher than I expected. With initial...

12 July 2020 415 3 View

Why does stresses evolve in only one element in Abaqus explicit?

I am trying to run my VUMAT code with a model in Abaqus/explicit. After that the run has been completed, I observed that the stresses have been evolved in only one element as shown in the attached...

27 May 2020 5,506 3 View

How to do baseline correction for DSC test data?

I have some raw DSC test data and I need to do baseline correction. I don't know how should I do it. I would appreciate it if someone can help me with this.

16 May 2020 8,273 7 View

Problem with nonlinear curve fitting?

Hello all, Actually, I am dealing with an issue that I can't get desirable results out of my curve-fitting problem. I used lsqcurvefit in the first place. Then, I added MultiStart to my code as...

30 April 2020 4,943 2 View

Tips on how to implement organic computing?

I'm researching the Self - Adaptive System of hospital systems.But I've had trouble figuring out how to do organic computing for self-adaptation in hospital systems.I am looking for an article or...

14 April 2020 1,743 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

How to choose mouse cell line to work on?

Hey, I am looking to start working on mouse cell line for some genomics experiments. I have the following requirements: 1) Normal chromosomes composition. 2) Possible to transfect. 3) Possible to...

03 January 2021 4,387 2 View

Which is the best gene editing tool among TALENs, ZFNs, CRISPR and HENs?

Since two decades we came across many tools to edit the desired gene and genome viz., clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), (2)...

27 December 2020 7,667 10 View