I have the lite version of ZEN ZEISS 3.7 software and I can't use the "Image Analysis" option because it is only available in the blue version. Is there another way to count the number of fluorescence signals per nuclei using ZEN lite?
the simple answer is no. There are however plenty of open-source software such as FIJI or Cellprofiler that can do the job and lot more. It is worthwhile to get acquainted with them.