How to model bubble in Ansys Fluent?

I am seeking to model a simple bubble growth/collapse in Ansys. I have tried using 2D, VOF with an initial patch air circle geometry surrounded by water. I include surface tension and make the air...

10 December 2020 9,113 3 View

Would ER NiCrMo-3 be the best option for weld overlay of carbon and low alloy steels?

Alloy 625 is the most popular alloy for weld overlay. Without considering the price factor, would NiCrMo-3 be the best technical option for weld overlay of API 5L and low alloy steels, in general,...

24 August 2020 2,478 4 View

What are the current best options to deplete rRNA in Arabidopsis?

I am interested in mRNAs only (also the ones from chloroplast). RNA input x RNA yielded approximately? Additional Kit used to concentrate RNAs + to get rid of other RNAs (tRNAs, etc...)? Thank...

01 June 2020 1,002 1 View

How can I prevent glycerol adsorption by PDMS after a couple of days in the incubator?

Cheers everyone I'm currently working with a microfabricated device composed by a thin PDMS sheet assembled onto a glycerol-coated glass slide. I need the PDMS to glide over the glass, hence I'm...

19 September 2018 4,705 3 View

Is it possible to recognize specific cell membrane potentials using nanoparticles?

I am looking for some type of nanoparticles I can use to detect cells with a specific membrane potential.

05 February 2018 8,757 2 View

How can we functionalize cyclic olefin for polyacrylamide hydrogel attachment?

Dear all, We are trying to make polyacrylamide hydrogels with tunable stiffness on cell culture plates with a cyclic olefin plastic bottom. The problem we have is that polyacrylamide gel does...

10 November 2017 9,155 3 View

Has the genetic process of speciation ever been modeled using the mathematical theory of bifurcation?

31 March 2017 5,325 3 View

What would be the best method for quantifying chlorhexidine digluconate?

Avaliable evidence only reports the chlorhexidine di aceate quantification. What avaliable methods could I use for chlorhexidine digluconate quantification. I deeply appreciate your support.

20 March 2017 1,094 5 View

Does anyone know how the transcriptomic profile of a patient with pancreatic cancer differs from that of a healthy (normal) person?

Pancreatic cancer is a worst type of cancer because it kills in a very short time, and there is currently no early detection method that I know where surgery can make a difference.

09 January 2017 2,001 3 View

How can I explain the fact that the size measured in SEM is different from the one I got by calculating crystallite size in XRD by Scherrer equation?

I have SEM images from ZnO nanoparticles whitch I've measured. Then I used the Scherrer equation to calculate the crystallite size but I can't get the result I expected, but a very smaller one.

13 April 2016 2,526 9 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View