I am trying to extract RNA from Aedes aegytpi to analyze gene expression in genes associated with the detoxification of insecticides. I am using the SV Total RNA Isolation System from Promega, but I got a very low yield of RNA (from 30 - 50 ng/ul). Can you share any tips or protocols or how have you solve low yield in RNA isolation from mosquitoes. Thank you very much.
Yamili Contreras-Perera
For RNA extraction you can use, Applied Biosystems® Arcturus® PicoPure® RNA isolation kit (Arcturus, biological Biosystems, USA), you can remove the DNA remnants with the Baseline-ZERO ™ DNase removal kit (Epicenter, Illumina) and the ribosomal RNA with the Ribo-Zero ™ rRNA removal kit (Human / Mouse / Rat) (Epicenter, Illumina) these protocols provide high RIN
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