Hi everyone
I want to learn by performing analysis methods about miniature IPSC, evoked IPSC, paired pulse depretion (PPD). That is why i need sample data sets. If you have these data, could you share it with me? Thank you so much!
The most accepted definition for RRP is " a small subset of the many vesicles in presynaptic bouton that is more readily released than other vesicles which are in the recycling and reserve pool",...
10 November 2019 7,607 4 View
I wanna perform homogenization for western blotting in rat brain tissue. But I wonder about which concentration protease inhibitory cocktail (Takara, cat no: 635673) I will use? or how can I...
05 June 2019 2,096 1 View
Does anyone know how the RG score is calculated? What does this score mean? What is the importance of this score? I think it is not a very important parameter for the academic career, would not be...
01 January 1970 8,960 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Would anyone be able to advise me... I have an RCT with a control and experimental group. Participants were recruited from one school (n=59). Participants were assessed using repeated measures on...
04 August 2024 4,040 6 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View