How to prevent clumping of EBV trasnfected lymphocytes?

I am culturing lymphocytes and they tend form visible clumps.. Does anybody know how to avoid this and if this is common ? I am new in culturing them. Thanks

04 May 2016 9,428 8 View

Contamination problems in 293 T cell culture. Any help ?

31 December 2015 1,872 17 View

Which antibody could I use against BRCA2 for Immunofluorescence?

I am looking for an Antibody to use in Immunofluorescence specific for BRCA2. I am not able to find a convincing IF image of BRCA2 by IF. Lot of scientists use the tagged version of BRCA2 in their...

08 September 2015 3,753 3 View

What are the ways to make the protein marker visible in a WB imager?

I have tried WB specific markers, but I would like to somehow image my protein markers while imaging. Is there a pen that would help ? Or are there other innovative ways?

04 May 2015 4,530 3 View

What is the conformation of the Holliday junction (HJ) inside the cell?

I am wondering if any of you have an idea about the conformation of HJ inside the cells - open or stacked? I am thinking it could be stacked, since that is a stable conformation than the open. But...

04 May 2014 5,610 2 View

Has anybody experienced protein sticking to the frits of the Superdex 200 column?

I am working on a human helicase protein and when I load them on a SEC column Superdex 200 (GE), I don't get a good recovery. In addition, when I load my protein-DNA complex into the same column...

31 December 2013 1,388 4 View

How can I stabilise a protein-DNA complex in isolation?

I have purified my protein-Holliday junction complex by SEC, but the purified complex is not stable. The protein dissociates from the DNA. I would want the complex to be stable to perform further...

31 December 2013 3,200 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View