Could you please kindly explain the chemistry/physics of DNA precipitation in the presence of PEG?

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Bobby L Boyanton

Agree with Oliver Krohn. In brief, precipitating DNA is based upon neutralizing cations that interact with the negatively charged phosphate backbone.

Subhojit Sen

PEG absorbs water very efficiently, and by that is able to pull H2O away from the water structure around B-DNA that keeps it in solution. Once that happens as explained by Oliver, the DNA basically falls out of solution. The same phenomenon can happen when adding Ethanol - which outcompetes the hydrogen bonds of water - thereby disrupting water structure around the DNA lattice.