10 Questions 26 Answers 0 Followers
Questions related from Peter V. Zolotukhin
Dear colleagues, I'm prettey used to preparing chitosan/TPP nanoparticles, but for my further studies, I'll need to disassemble them. Surely I can use enzymatic digestion of chitosan, but I'd...
22 April 2017 4,248 3 View
Dear colleagues, I've recently found an interesting paper (PMID 21763280) on the phenotypes of necrotic and apoptotic cells. As seen from Figure 1, cells treated with TNF and CHX undergo either...
14 August 2015 9,093 2 View
Dear colleagues, so the question is in the title... Background: we've spent a few days trying to isolate RNA from an adherent cell culture grown in a 6-well plate. Isolating RNA from various...
25 March 2015 9,255 11 View
Dear colleagues, could you please tell me if there are any glycine and serine conjugation reagents that are applicable in vivo? For example, like phenylbutyrate is a conjugator for glutamine.
20 February 2015 3,596 1 View
Dear colleagues, could you please tell me if you've ever met a reliable and not too expensive instrument for assessing cell viability (the trypan blue exclusion method preferred) which runs on...
06 February 2015 6,772 4 View
PEG DNA precipitation
29 August 2014 9,770 2 View
Dear colleagues, I cannot find a comprehensive answer on the question: how does, if at all, necrosis actually kill cells surrounding necrotic are? Is the necrotic spread caused by an extracellular...
29 August 2014 4,137 3 View
Dear colleagues, I'm planning an experiment, and to buy an appropriate amount of reagents, I need to find out the number of HepG2 cells per 1 cm^2 of a culturing flask. Please help.
25 July 2014 4,248 5 View
Dear colleagues, how many scrambled siRNAs do you use in case when you either target single gene with two or more siRNAs or target multiple genes with multiple siRNAs?
03 February 2014 3,548 6 View
Do you use the passive reference dye in DNA-binding-dye type real-time PCR?
24 August 2013 6,412 6 View
