How to use the results of Co-IP for protein quantitative difference calculation?

I did a Co-IP MS with two samples and got the LFQ data from MaxQuant, and I wanted to normalize it by an internal reference protein. After I divide the LFQ of the other protein by the LFQ of the...

18 January 2021 5,419 4 View

I have tissue sections on Superfrost Slides (NOT Plus) and they fall off during IF. Any suggestion?

Hi, I have OCT embedded tissue cut on Superfrost slides. However these slides are not Superfrost Plus, so apparently they are not coated. During IF all my sections are lost. How can I save them?...

18 January 2021 5,256 2 View

Does someone had trouble with fixable life/dead in endometrial flow cytometry?

Hi. I am doing flow cytometry of endometrial cells (read epithelial and fibroblast). The antibodies I need to use are both intracellular. In the same time I want to get only life cells. Thus I...

07 January 2021 6,473 3 View

How to purify cyclic peptides?

Hello, We have synthesized the cyclic peptide (D-Glu(OtBu)-L-Lys(Alloc)-D-Glu(OtBu)-L-Ala)2. LC-MS shows a mixture of linear and cyclic peptides. There is only one peak by HPLC (reverse phase,...

21 July 2020 2,237 1 View

What are the storage conditions that prevent aggregation in B-amyloid peptids?

We are expressing B-amyloid peptids and after purification we are having some problems with the aggregation of the samples. I'm wondering if there are optimal conditions of storage that dont...

07 March 2020 9,366 3 View

How can I disaggregate proteins?

I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...

24 February 2020 7,437 4 View

What is the reason why poly-D-galacturonic acid is water insoluble and pectin is water soluble?

Pectin is mainly composed of polyagalacturonic acid (>70%) and yet is water soluble, whereas the pure acid is completely insoluble in water, even upon heating. The degree of estherification...

28 October 2019 9,552 5 View

DNA precipitation without ethanol?

Is there a way of precipitating DNA from water without using ethanol? for example, mixing DNA with glycogen and ammonium acetate and then spinning it long enough so the bound glycogen+dna would...

22 August 2019 5,501 6 View

SDM multiple substitution?

I would like to substitute 3 aa (9 nt in total) with SDM using a single large primer to carry out multiple substitutions in a single run. I have designed the primers using the QuikChange Primer...

08 April 2019 9,620 3 View

Looking for lab specialized in service analysis on rats serum?

Hi, i m looking for a lab specialized on this kind of analysis ALT/AST ADH/ALDH CYP2E1 on rats serum, in service

01 May 2018 9,498 1 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View