I would like to study the mutations on CD133 gene in colorectal cancer. Kindly let me know what primers I can use
You could retrieve the CD133 sequence from NCBI and design primers using Primer 3 or primer blast tool of NCBi. Alternatively please refer to these articles,
1. Gopisetty, G et al. 'Epigenetic Regulation Of CD133/PROM1 Expression In Glioma Stem Cells By Sp1/Myc And Promoter Methylation'. Oncogene 32.26 (2012): 3119-3129.
2. Mak, A. B. et al. 'CD133 Protein N-Glycosylation Processing Contributes To Cell Surface Recognition Of The Primitive Cell Marker AC133 Epitope'. Journal of Biological Chemistry 286.47 (2011): 41046-41056.
3. Tabu, Kouichi et al. 'Analysis Of An Alternative Human CD133 Promoter Reveals The Implication Of Ras/ERK Pathway In Tumor Stem-Like Hallmarks'. Molecular Cancer 9.1 (2010): 39.
Alternatively you can search via primer bank. they will provide you validated primer sequence for both general as well as real time PCR.
Good Luck
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