Punith B D

6 Questions 9 Answers 0 Followers

Questions related from Punith B D

Which is the best method/algorithm for phylogenetic comparison of protein coding sequences (ORF) and amino acid sequences?

I need compare a particular protein coding (nucleotide) sequence and amino acid sequence with vertebrates and non-vertebrates and understand the mechanisms of evolution and relatedness using...

12 December 2016 3,494 3 View

Would someone please share a protocol for selective removal of Hemoglobin from hemolysates or erythrocyte lysates?

I need to remove the hemoglobin from the whole blood lysates and erythrocyte lysates for Mass spectrometry based proteomics. Since hemoglobin is most abundant protein which will mask and...

01 January 2016 6,801 5 View

How to annotate the nucleotide sequences in terms of KEGG Orthology (KO)?

I have a RNA-Seq data in which I have to construct the KEGG Pathway for DGE between two samples. I also have nucleotide sequences (contigs) in the fasta format (.fa) file. Kindly tell me how to...

05 May 2015 9,312 5 View

Is their any bioinformatic tool for mining transcription factors from RNA-Seq data and constructing the network of pathways associated?

I need to understand the dynamics of transcription factor expression in the RNA-Seq data. Is there any tool to identify the transcription factors from the RNA-Seq data. Also suggest me tools for...

04 April 2015 6,384 4 View

Does anyone have a protocol for in-vitro differentiation of bone marrow mesenchymal stem cells to hepatocytes?

So far I get the protocols and the research articles which describe the differentiation of mesenchymal stem cells into hepatocyte like cells, but not hepatocytes exactly. Also please suggest me...

06 June 2014 1,534 1 View

What is a good culture medium for establishing primary hepatocytes? Why should phenol red not be used in hepatocyte culture?

If anyone has done primary liver cell culture please suggest which is the good culture medium for primary hepatocytes and also tell me prospects for using DMEM or DMEM-Ham's F12. Also please...

06 June 2014 7,553 13 View