Prashant Akila

6 Questions 5 Answers 0 Followers

Questions related from Prashant Akila

Which hybridisation chamber can be used for Fluorescent in-situ hybridisation technique?

I require the details like the company and model of the hybridisation chamber that can be used for performing FISH

05 May 2018 6,901 2 View

What are the specifications to be looked for when procuring the fluorescent microscope with karyotyping and FISH software?

We are in a process of setting up a cytogenetic laboratory in our institute for the diagnosis of inherited disorders. We have approached olympus and carl zies for the same. Kindly let me know what...

05 May 2018 8,345 1 View

Are there any guidelines while designing a primer to study polymorphism of a gene?

I would like to study the polymorphism of tgf beta 1 gene. Can I chose any primer for amplification of a segment of this gene and study the polymorphism or should I amplify the whole gene.

03 March 2016 6,313 2 View

Can we use the same house keeping gene primers for human and mouse samples?

We have primers for human GAPDH gene. Can I use these primers when I perform real time PCR with mouse samples. Kindly please advice...

06 June 2015 7,069 2 View

Could you please suggest me the primer sequence for CD133 gene?

I would like to study the mutations on CD133 gene in colorectal cancer. Kindly let me know what primers I can use

04 April 2015 8,607 2 View

What are the different ways to check if our primer pair is specific to a certain species of bacteria?

We have designed primers for specific bacterial species. The PCR is showing good results by amplifying only the specific bacteria with no amplification with other bacteria. How can I further...

01 January 1970 4,645 11 View