How can we perform the skin sampling from psoriasis-like mice model for immunohistochemistry?

Hello everyone ! I am willing to sample the mouse skin for immunohistochemistry analysis from the mouse having psoriasis-like symptoms in dorsal skin layer. Could you please help me how should I...

07 August 2017 4,066 1 View

Could you please suggest me for protein quantification using nanodrop 2000c?

I was using Bradfords reagent for protein quantification in microplate reader. The problem I faced was I got variations in house keeping gene. The pity is that some sample even did not...

08 September 2016 6,826 1 View

How can we determine the phenotype of activated microglia through its morphology?

Hello ! I am working on microglia phenotype study following focal cerebral ischemic challenge in mice. Through the analysis of different biochemical markers (by qPCR, and western blot) we can...

08 September 2016 2,879 9 View

Is it okay to use water while diluting cDNA or primers?

Previously we used to use RNAse free water (DEPC water) for all genetic experiments that is RNA extraction, cDNA synthesis, semi-PCR or RT-PCR. Recently we use DEPC water only for RNA work. For...

03 April 2016 3,781 5 View

Could you please suggest me the mounting medium/solution for cells ?

I am using VECTA SHELD mounting solution to mount my cells growing in coverslips. I wish to fix my cells using fixative solution and for that I have to use another mounting solution. And moreover,...

03 April 2016 9,588 2 View

Could you please let me know the size and length of monofilament for tMCAo induction in C57/BL6 male mice of 8 weeks?

I am currently using CD-1 mice of 7 week age for MCAo purpose and I am about to perform the tMCAo in C57/BL6 mice. Could you please let me know the mono-filament size (diameter) for MCAo and I...

02 March 2016 1,208 7 View

Does all experimental interventions are neuroprotective following stroke?

Could you please provide me some article which showed improvement of neurological deficit without change in infarct volume or vice versa after drug treatment flowing stroke induction in rodents.

02 March 2016 7,036 3 View

Have you ever experienced the mismatching between infarct volume and neurological score following MCAo ?

01 February 2016 7,414 2 View

How to extract proteins from formaldehyde fixed frozen tissue section (any kits) ?

I am planning to extract the proteins for western blot purpose from the fixed brain tissue sections. Brains tissue were fixed in 4% para formaldehyde and frozen at -80 degree Celsius. Do you know...

10 November 2015 2,837 2 View

What would be the best marker for microglia in cerebral ischemia ?

We are using iba1 as the marker for the activation and proliferation study of microglia following focal cerebral ischemia. However iba1 stains both macrophages and microglia thus creating...

09 October 2015 2,506 8 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View