Could someone share their protocol and exprience in isolating synaptosomal and non-synaptosomal mitochondria from rodent small brain areas?

Mercedes Pérez-Hernández @Mercedes_Perez-Hernandez

02 February 2019 1 2K Report

Hello everyone. I am looking for a good protocol for isolating mitochondria from brain, but I want to separate synaptosomal mitochondria from non-synaptosomal mitochondria in hippocampus. I know some protocols for this, but they use the whole brain or some areas bigger than hippocampus. I absolutely know that I must pool several hippocampus, but I want to know wheter it is technically possible or not. Could someone share with me his experience? Thanks!

Yogesh Kanna Sathyamoorthy

Dear Pérez-Hernández

I have attached a protocol for synaptoneurosome isolation along with a reference paper. This protocol doesnt need neither ultracentrifuge nor percoll.

Hope this is helpful for the project.

Regards,

Yogesh Kanna Sathyamoorthy

Badges
Science topic

More Mercedes Pérez-Hernández's questions See All

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View

I would like. How they can be simulated the deformational processes of valvular aortic stents??

We have a projet about TAVI

22 December 2020 7,729 1 View

Using multiple linear regression to solve a multiple-way ANOVA problem?

Specifically, I want to know if I can use multiple linear regression to predict a response value 'y' from it's associated "treatment" or "group" averages in several variables. Let's say:...

04 November 2020 632 19 View

PRECIPITATION MODELING OF V CARBIDE IN Mn STEEL?

HELLO DEAR COLLEGUES, Is there anybody who have acces to MATCALC software that help me modelling the precipitation phenomena in a medium Mn steel microalloyed with 0.15%wt. of V? The expected...

05 October 2020 5,914 3 View

Does the molecular weight of PVP matter in CTAB buffer for DNA and RNA extraction?

I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about...

05 September 2020 7,488 4 View

Why does the 2D peak intensity in a graphene Raman spectrumdecrease with the number of layers?

If we compare a Raman spectrum of monolayer graphene and bilayer or fewlayer graphene, we will see that the 2D peak intensity decreases (relative to the G peak intensity) for the later cases. I...

24 July 2020 907 3 View

One-way ANOVa for non-homogeneous and not normal data?

I have a data set that has 3 groups and a single variable. Under normal circumstances, a one-way ANOVA would be used for comparisons between my three groups. HOWEVER I ran a Shapiro-Wilk test for...

22 July 2020 6,061 7 View

How to derive the expression for the View Factor of a configuration of two bodies, starting from the general case ?

Hi all : Please, I need some help on Heat Radiation Transfer: How I can derive the formula of the View Factor for a particular configuration, in a way of ending with an expression for the VF as...

15 May 2020 4,929 6 View

Radiation Heat Transfer between a Hot body and a real Surface ?

Hi all , How can I consider the Optical properties of a surface into the Stefan-Boltzmann law for a problem of Radiation Heat Transfer between a hot Body and a real Surface (namely, a surface...

15 May 2020 9,449 8 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

BrdU staining issue?

Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...

13 January 2021 9,449 0 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View