Could somebody recommend a method for molecular cloning for expression from fungi?

More Ayşe Üstün's questions See All

Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

Subjects number in SEM for experimental design?

For an experimental design ( one experiment and one control group), at least or minimum, how many subjects need to be included in the groups in SEM?

08 January 2024 8,210 3 View

Article Removal Request?

07 November 2023 4,567 2 View

How can i turn any survey result into mathematical modelling ?

What I want to ask is this: I have a survey and its analysis. I want to build a mathematical model using these analyses. how can I do it

04 September 2023 9,331 3 View

Why do we use sodium cyanide in this protocol of sod assay? What happen if i don't use?

Superoxide Dismutase Assay Method Activity of SOD has been determined in two ways: 1. Inhibition by the enzyme of an O2- dependent reaction. 2. Pulse radiolytic methods (Rigo et al. 1975) See:...

14 August 2023 3,191 1 View

If X predicts Y, and Y predicts Z; does that reflect an indirect relationship?

Hello dear researchers In my study, I have 5 independent variables and 1 dependent variable. In multiple regression analysis, not all my IVs significantly predicted my DV. However, most of my IVs...

27 October 2022 8,623 4 View

Has anyone used siRNA from Synbio Technologies before?

Hello, I am planning to do gene knockdown using siRNA. Has anyone used siRNA from Synbio Technologies before? If so, what are your thoughts?

19 March 2022 4,655 0 View

How can I transform effect sizes (cohen's d) before running a meta-analysis in R?

I'm using R to run a meta-analysis (using Robust Variance Estimation) of Cohen's d effect sizes. What transformations do I need to do and how do I do them in R? Does anyone have access to online...

13 September 2021 7,239 3 View

Why Native PAGE gel is empty?

Can you help me. I am a student Biology Department. I upload and ran the Native PAGE gel. After 3 hours, even the markers were not visible on the gel. I turned it off and did the painting, but the...

24 June 2021 2,668 2 View

What is the optimal candidal biofilm adhesion time to determine at the candida albicans biofilm inhibiting effect of a substance?

candida albicans biofilm

22 May 2021 9,688 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View